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Recombinant DNA Technology

Recombinant DNA Technology
Which type of restriction enzymes do not usually require ATP?

Type II
Type IV
Type III
Type I

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Recombinant DNA Technology
In order to insert a foreign gene into a plasmid, both must __________

have identical DNA sequences
originate from the same type of cell
be of the same length
be cut by the same restriction enzyme

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Recombinant DNA Technology
Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

All of these
both techniques rely on a constant charge to mass ratio
both techniques utilize the sieving properties of gels
in both cases molecules migrate to the anode

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Recombinant DNA Technology
First discovered, Type II restriction endonuclease was

EcoRI
Hind II
Eco K
Hinf I

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Recombinant DNA Technology
Which of the following genetic diseases would be amenable to genetic engineering?

Down's syndrome
Cri du Chat
Muscular dystrophy
Cystic fibrosis

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Recombinant DNA Technology
The unpaired nucleotides produced by the action of restriction enzymes are referred to have

single strands
restriction fragments
sticky ends
ligases

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