Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type II Type III Type IV Type I Type II Type III Type IV Type I ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is correct in terms of determination of location of genetic traits? None of these Protein-coding genes are always associated with a restriction pattern Restriction sites allow DNAs to be digested Known protein coding sequences are too far apart to allow linkage determination for most new genes None of these Protein-coding genes are always associated with a restriction pattern Restriction sites allow DNAs to be digested Known protein coding sequences are too far apart to allow linkage determination for most new genes ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends restriction fragments single strands ligases sticky ends restriction fragments single strands ligases ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid always contains an origin of replication usually contains one or more restriction sites is a circular DNA molecule All of these always contains an origin of replication usually contains one or more restriction sites is a circular DNA molecule All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Mbo I & Sau 3A Msp I & HPA II Eco RI & MspI Sau 3A & Bam HI Mbo I & Sau 3A Msp I & HPA II Eco RI & MspI Sau 3A & Bam HI ANSWER DOWNLOAD EXAMIANS APP