Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because both techniques rely on a constant charge to mass ratio All of these both techniques utilize the sieving properties of gels in both cases molecules migrate to the anode both techniques rely on a constant charge to mass ratio All of these both techniques utilize the sieving properties of gels in both cases molecules migrate to the anode ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of these genes codes for a protein that plays a role in white blood cell function? MPO RP13 DCP1 GLUT4 MPO RP13 DCP1 GLUT4 ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following can be diagnosed by amniocentesis? Cystic Fibrosis Sickle cell anemia Down's syndrome All of these Cystic Fibrosis Sickle cell anemia Down's syndrome All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have single strands restriction fragments sticky ends ligases single strands restriction fragments sticky ends ligases ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are short sequences that will hybridize only to a specific base sequence PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene ANSWER DOWNLOAD EXAMIANS APP
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