Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of these genes codes for a protein that plays a role in growth? SCLC6A4 DCP1 KRTHA1 GH1 SCLC6A4 DCP1 KRTHA1 GH1 ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because both techniques utilize the sieving properties of gels All of these both techniques rely on a constant charge to mass ratio in both cases molecules migrate to the anode both techniques utilize the sieving properties of gels All of these both techniques rely on a constant charge to mass ratio in both cases molecules migrate to the anode ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following genetic diseases would be amenable to genetic engineering? Cystic fibrosis Muscular dystrophy Down's syndrome Cri du Chat Cystic fibrosis Muscular dystrophy Down's syndrome Cri du Chat ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are short sequences that will hybridize only to a specific base sequence PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology First discovered, Type II restriction endonuclease was Eco K EcoRI Hinf I Hind II Eco K EcoRI Hinf I Hind II ANSWER DOWNLOAD EXAMIANS APP
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