Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of these restriction enzymes produce blunt ends? XhoI SaII EcoRV HindIII XhoI SaII EcoRV HindIII ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The TP53 gene of chromosome 17 codes for a protein __________ . that plays a role in the digestive process that is like a white blood cell protein involved in glucose transport involved in the regulation of the cell cycle that plays a role in the digestive process that is like a white blood cell protein involved in glucose transport involved in the regulation of the cell cycle ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Mbo I & Sau 3A Eco RI & MspI Sau 3A & Bam HI Msp I & HPA II Mbo I & Sau 3A Eco RI & MspI Sau 3A & Bam HI Msp I & HPA II ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains DNA segments for the regulation of mRNA production always contains an origin of replication usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains DNA segments for the regulation of mRNA production always contains an origin of replication ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved different length fragments of DNA resulting from loss or gain of a restriction site All of these an Eco RI cuts DNA at a different sequence than Hind III cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved different length fragments of DNA resulting from loss or gain of a restriction site All of these an Eco RI cuts DNA at a different sequence than Hind III ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS