Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved All of these different length fragments of DNA resulting from loss or gain of a restriction site an Eco RI cuts DNA at a different sequence than Hind III cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved All of these different length fragments of DNA resulting from loss or gain of a restriction site an Eco RI cuts DNA at a different sequence than Hind III ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following genetic diseases would be amenable to genetic engineering? Cystic fibrosis Down's syndrome Cri du Chat Muscular dystrophy Cystic fibrosis Down's syndrome Cri du Chat Muscular dystrophy ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type II Type I Type III Type IV Type II Type I Type III Type IV ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The RP13 gene of chromosome 17 codes for a protein __________ . that is a component of hair and nails involved in eye development involved in glucose transport involved in the determination of personality that is a component of hair and nails involved in eye development involved in glucose transport involved in the determination of personality ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because both techniques utilize the sieving properties of gels All of these in both cases molecules migrate to the anode both techniques rely on a constant charge to mass ratio both techniques utilize the sieving properties of gels All of these in both cases molecules migrate to the anode both techniques rely on a constant charge to mass ratio ANSWER DOWNLOAD EXAMIANS APP
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