Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends single strands restriction fragments ligases sticky ends single strands restriction fragments ligases ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Restriction enzymes are named for the person who discovered the bacterium they are derived from the viral DNA that they attack None of these the person who discovered the bacterium they are derived from the viral DNA that they attack None of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is correct in terms of determination of location of genetic traits? Restriction sites allow DNAs to be digested Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes None of these Restriction sites allow DNAs to be digested Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes None of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology X-rays cause the formation of thymine dimers None of these heat ionization of water in the cell the formation of thymine dimers None of these heat ionization of water in the cell ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring ANSWER DOWNLOAD EXAMIANS APP
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