Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Restriction enzymes All of these cut DNAs producing a blunt end cut DNA in a staggered fashion protect bacteria from viral infection All of these cut DNAs producing a blunt end cut DNA in a staggered fashion protect bacteria from viral infection ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ . has occurred seems unlikely would be of no concern if it occurred. can never occur has occurred seems unlikely would be of no concern if it occurred. can never occur ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ . heterosis genetic drift inbreeding assortative mating heterosis genetic drift inbreeding assortative mating ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS