Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type II Type IV Type I Type III Type II Type IV Type I Type III ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains DNA segments for the regulation of mRNA production always contains an origin of replication usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains DNA segments for the regulation of mRNA production always contains an origin of replication ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology First discovered, Type II restriction endonuclease was Eco K EcoRI Hind II Hinf I Eco K EcoRI Hind II Hinf I ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Sau 3A & Bam HI Mbo I & Sau 3A Msp I & HPA II Eco RI & MspI Sau 3A & Bam HI Mbo I & Sau 3A Msp I & HPA II Eco RI & MspI ANSWER DOWNLOAD EXAMIANS APP
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