Recombinant DNA Technology
The TP53 gene of chromosome 17 codes for a protein __________ .

involved in the regulation of the cell cycle
involved in glucose transport
that is like a white blood cell protein
that plays a role in the digestive process

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence
copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)

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Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus

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