Recombinant DNA Technology
The TP53 gene of chromosome 17 codes for a protein __________ .

that is like a white blood cell protein
involved in glucose transport
that plays a role in the digestive process
involved in the regulation of the cell cycle

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)
short sequences that will hybridize only to a specific base sequence
mutagenized copies of a gene

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Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
always contains an origin of replication
All of these
usually contains a gene that confers antibiotic resistance to the bacterial host

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