Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
different length fragments of DNA resulting from loss or gain of a restriction site
All of these

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Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus

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Recombinant DNA Technology
Isoschizomers recognize

different recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and different recognition sequence
same recognition site and recognition sequence

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
All of these
always contains DNA segments for the regulation of mRNA production
usually contains a gene that confers antibiotic resistance to the bacterial host

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