Recombinant DNA Technology An example of a restriction fragment length polymorphism is an Eco RI cuts DNA at a different sequence than Hind III All of these different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved an Eco RI cuts DNA at a different sequence than Hind III All of these different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A human cDNA library contains DNA for virtually all of the human proteins in vectors cannot be used to obtain human genes because it would be radioactive contains DNA for specific human proteins None of these contains DNA for virtually all of the human proteins in vectors cannot be used to obtain human genes because it would be radioactive contains DNA for specific human proteins None of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. negative ... positive long ... short basic ... acidic positive to negative negative ... positive long ... short basic ... acidic positive to negative ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid is a circular DNA molecule All of these usually contains one or more restriction sites always contains an origin of replication is a circular DNA molecule All of these usually contains one or more restriction sites always contains an origin of replication ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
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