Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
different length fragments of DNA resulting from loss or gain of a restriction site
All of these
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

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Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines

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