Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

short sequences that will hybridize only to a specific base sequence
mutagenized copies of a gene
copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)

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Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors
cannot be used to obtain human genes because it would be radioactive
None of these

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