Recombinant DNA Technology
Isoschizomers recognize

same recognition site and recognition sequence
same recognition sequence but different recognition site
different recognition site and different recognition sequence
same recognition site and different recognition sequence

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Recombinant DNA Technology
A human cDNA library

None of these
contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors
cannot be used to obtain human genes because it would be radioactive

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
different length fragments of DNA resulting from loss or gain of a restriction site
All of these
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

short sequences that will hybridize only to a specific base sequence
mutagenized copies of a gene
PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted

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