Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines

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