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Recombinant DNA Technology

Recombinant DNA Technology
Which of these restriction enzymes produce blunt ends?

HindIII
EcoRV
SaII
XhoI

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Recombinant DNA Technology
Which of the following pair will produce complementary sticky ends?

Sau 3A & Bam HI
Msp I & HPA II
Mbo I & Sau 3A
Eco RI & MspI

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Recombinant DNA Technology
The unpaired nucleotides produced by the action of restriction enzymes are referred to have

restriction fragments
sticky ends
ligases
single strands

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Recombinant DNA Technology
Restriction enzymes

cut DNA in a staggered fashion
cut DNAs producing a blunt end
protect bacteria from viral infection
All of these

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Recombinant DNA Technology
First discovered, Type II restriction endonuclease was

Eco K
Hinf I
Hind II
EcoRI

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Recombinant DNA Technology
Plant transformation can be obtained by

shooting DNA into plant cells with a gun
infecting plants with a tobacco mosaic virus
using the E. coli bacterium to infect plant roots
combining plant and animal cells in culture

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