Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive
contains DNA for virtually all of the human proteins in vectors
None of these

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Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence
PCR-amplified variable numbers of tandem repeats (VNTRs)

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