Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
different length fragments of DNA resulting from loss or gain of a restriction site
All of these

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

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Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive
None of these
contains DNA for virtually all of the human proteins in vectors

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