Recombinant DNA Technology Which of these restriction enzymes produce blunt ends? SaII XhoI HindIII EcoRV SaII XhoI HindIII EcoRV ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are PCR-amplified variable numbers of tandem repeats (VNTRs) mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid always contains an origin of replication is a circular DNA molecule All of these usually contains one or more restriction sites always contains an origin of replication is a circular DNA molecule All of these usually contains one or more restriction sites ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is not true of small inbreeding populations? Mutation is increased There is an increased incidence of recessive diseases Alleles may become fixed They tend to loose genetic diversity Mutation is increased There is an increased incidence of recessive diseases Alleles may become fixed They tend to loose genetic diversity ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ originate from the same type of cell be cut by the same restriction enzyme be of the same length have identical DNA sequences originate from the same type of cell be cut by the same restriction enzyme be of the same length have identical DNA sequences ANSWER DOWNLOAD EXAMIANS APP
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