Recombinant DNA Technology
Isoschizomers recognize

different recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and different recognition sequence
same recognition site and recognition sequence

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern
None of these

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