Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
An expression vector

usually contains a gene that confers antibiotic resistance to the bacterial host
All of these
always contains an origin of replication
always contains DNA segments for the regulation of mRNA production

ANSWER DOWNLOAD EXAMIANS APP