Recombinant DNA Technology Which of these restriction enzymes produce blunt ends? XhoI HindIII EcoRV SaII XhoI HindIII EcoRV SaII ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is correct in terms of determination of location of genetic traits? Protein-coding genes are always associated with a restriction pattern None of these Known protein coding sequences are too far apart to allow linkage determination for most new genes Restriction sites allow DNAs to be digested Protein-coding genes are always associated with a restriction pattern None of these Known protein coding sequences are too far apart to allow linkage determination for most new genes Restriction sites allow DNAs to be digested ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology X-rays cause ionization of water in the cell heat the formation of thymine dimers None of these ionization of water in the cell heat the formation of thymine dimers None of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector always contains an origin of replication usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains DNA segments for the regulation of mRNA production always contains an origin of replication usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains DNA segments for the regulation of mRNA production ANSWER DOWNLOAD EXAMIANS APP
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