Recombinant DNA Technology
The RP13 gene of chromosome 17 codes for a protein __________ .

that is a component of hair and nails
involved in glucose transport
involved in eye development
involved in the determination of personality

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Restriction sites allow DNAs to be digested
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Protein-coding genes are always associated with a restriction pattern
None of these

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines

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Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors
None of these
cannot be used to obtain human genes because it would be radioactive

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