Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. basic ... acidic negative ... positive long ... short positive to negative basic ... acidic negative ... positive long ... short positive to negative ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have single strands restriction fragments ligases sticky ends single strands restriction fragments ligases sticky ends ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because both techniques utilize the sieving properties of gels both techniques rely on a constant charge to mass ratio in both cases molecules migrate to the anode All of these both techniques utilize the sieving properties of gels both techniques rely on a constant charge to mass ratio in both cases molecules migrate to the anode All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is correct in terms of determination of location of genetic traits? Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes Restriction sites allow DNAs to be digested None of these Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes Restriction sites allow DNAs to be digested None of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved All of these an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
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