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Recombinant DNA Technology

Recombinant DNA Technology
What enzyme forms covalent bonds between restriction fragments?

DNA ligase
DNA polymerase
DNA helicase
DNA primase

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Recombinant DNA Technology
Restriction enzymes

All of these
cut DNAs producing a blunt end
protect bacteria from viral infection
cut DNA in a staggered fashion

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Recombinant DNA Technology
Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

All of these
both techniques rely on a constant charge to mass ratio
both techniques utilize the sieving properties of gels
in both cases molecules migrate to the anode

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Recombinant DNA Technology
The unpaired nucleotides produced by the action of restriction enzymes are referred to have

sticky ends
single strands
restriction fragments
ligases

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Recombinant DNA Technology
A plasmid

All of these
always contains an origin of replication
is a circular DNA molecule
usually contains one or more restriction sites

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Recombinant DNA Technology
Which of the following techniques can be used to determine the defective gene and for developing cancer?

Southern blot
Eastern blot
Western blot
Northern blot

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