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Recombinant DNA Technology

Recombinant DNA Technology
What enzyme forms covalent bonds between restriction fragments?

DNA primase
DNA ligase
DNA polymerase
DNA helicase

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Recombinant DNA Technology
Plant transformation can be obtained by

using the E. coli bacterium to infect plant roots
shooting DNA into plant cells with a gun
combining plant and animal cells in culture
infecting plants with a tobacco mosaic virus

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Recombinant DNA Technology
Which of these restriction enzymes produce blunt ends?

EcoRV
XhoI
HindIII
SaII

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Recombinant DNA Technology
A plasmid

always contains an origin of replication
usually contains one or more restriction sites
All of these
is a circular DNA molecule

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Recombinant DNA Technology
Which of the following genetic diseases would be amenable to genetic engineering?

Muscular dystrophy
Cri du Chat
Down's syndrome
Cystic fibrosis

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Recombinant DNA Technology
The unpaired nucleotides produced by the action of restriction enzymes are referred to have

ligases
restriction fragments
sticky ends
single strands

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