Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA ligase DNA helicase DNA primase DNA polymerase DNA ligase DNA helicase DNA primase DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type II Type I Type IV Type III Type II Type I Type IV Type III ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ originate from the same type of cell be cut by the same restriction enzyme have identical DNA sequences be of the same length originate from the same type of cell be cut by the same restriction enzyme have identical DNA sequences be of the same length ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A human cDNA library None of these contains DNA for virtually all of the human proteins in vectors contains DNA for specific human proteins cannot be used to obtain human genes because it would be radioactive None of these contains DNA for virtually all of the human proteins in vectors contains DNA for specific human proteins cannot be used to obtain human genes because it would be radioactive ANSWER DOWNLOAD EXAMIANS APP
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