Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA helicase DNA polymerase DNA primase DNA ligase DNA helicase DNA polymerase DNA primase DNA ligase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is different length fragments of DNA resulting from loss or gain of a restriction site an Eco RI cuts DNA at a different sequence than Hind III All of these cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved different length fragments of DNA resulting from loss or gain of a restriction site an Eco RI cuts DNA at a different sequence than Hind III All of these cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. positive to negative long ... short basic ... acidic negative ... positive positive to negative long ... short basic ... acidic negative ... positive ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The RP13 gene of chromosome 17 codes for a protein __________ . involved in the determination of personality involved in eye development involved in glucose transport that is a component of hair and nails involved in the determination of personality involved in eye development involved in glucose transport that is a component of hair and nails ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid All of these is a circular DNA molecule always contains an origin of replication usually contains one or more restriction sites All of these is a circular DNA molecule always contains an origin of replication usually contains one or more restriction sites ANSWER DOWNLOAD EXAMIANS APP
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