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Recombinant DNA Technology

Recombinant DNA Technology
What enzyme forms covalent bonds between restriction fragments?

DNA helicase
DNA polymerase
DNA ligase
DNA primase

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Recombinant DNA Technology
Plant transformation can be obtained by

combining plant and animal cells in culture
infecting plants with a tobacco mosaic virus
shooting DNA into plant cells with a gun
using the E. coli bacterium to infect plant roots

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Recombinant DNA Technology
Restriction enzymes

cut DNAs producing a blunt end
cut DNA in a staggered fashion
All of these
protect bacteria from viral infection

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Recombinant DNA Technology
The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

would be of no concern if it occurred.
has occurred
seems unlikely
can never occur

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Recombinant DNA Technology
Which of the following techniques can be used to determine the defective gene and for developing cancer?

Northern blot
Eastern blot
Southern blot
Western blot

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Recombinant DNA Technology
Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

both techniques utilize the sieving properties of gels
All of these
in both cases molecules migrate to the anode
both techniques rely on a constant charge to mass ratio

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