Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA polymerase DNA primase DNA ligase DNA helicase DNA polymerase DNA primase DNA ligase DNA helicase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Isoschizomers recognize different recognition site and different recognition sequence same recognition sequence but different recognition site same recognition site and different recognition sequence same recognition site and recognition sequence different recognition site and different recognition sequence same recognition sequence but different recognition site same recognition site and different recognition sequence same recognition site and recognition sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ originate from the same type of cell have identical DNA sequences be of the same length be cut by the same restriction enzyme originate from the same type of cell have identical DNA sequences be of the same length be cut by the same restriction enzyme ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type II Type IV Type I Type III Type II Type IV Type I Type III ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines ANSWER DOWNLOAD EXAMIANS APP
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