Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA helicase DNA polymerase DNA ligase DNA primase DNA helicase DNA polymerase DNA ligase DNA primase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Restriction enzymes All of these cut DNA in a staggered fashion protect bacteria from viral infection cut DNAs producing a blunt end All of these cut DNA in a staggered fashion protect bacteria from viral infection cut DNAs producing a blunt end ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ . genetic drift inbreeding heterosis assortative mating genetic drift inbreeding heterosis assortative mating ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type IV Type II Type I Type III Type IV Type II Type I Type III ANSWER DOWNLOAD EXAMIANS APP
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