Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA polymerase DNA helicase DNA primase DNA ligase DNA polymerase DNA helicase DNA primase DNA ligase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Restriction enzymes are named for the bacterium they are derived from the viral DNA that they attack None of these the person who discovered the bacterium they are derived from the viral DNA that they attack None of these the person who discovered ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A human cDNA library cannot be used to obtain human genes because it would be radioactive contains DNA for virtually all of the human proteins in vectors None of these contains DNA for specific human proteins cannot be used to obtain human genes because it would be radioactive contains DNA for virtually all of the human proteins in vectors None of these contains DNA for specific human proteins ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Eastern blot Western blot Northern blot Southern blot Eastern blot Western blot Northern blot Southern blot ANSWER DOWNLOAD EXAMIANS APP