Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines
mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence

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Recombinant DNA Technology
A human cDNA library

contains DNA for virtually all of the human proteins in vectors
None of these
contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive

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