Recombinant DNA Technology
Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

both techniques utilize the sieving properties of gels
All of these
in both cases molecules migrate to the anode
both techniques rely on a constant charge to mass ratio

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
All of these
an Eco RI cuts DNA at a different sequence than Hind III
different length fragments of DNA resulting from loss or gain of a restriction site

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Recombinant DNA Technology
A human cDNA library

contains DNA for virtually all of the human proteins in vectors
cannot be used to obtain human genes because it would be radioactive
contains DNA for specific human proteins
None of these

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