Recombinant DNA Technology
Which of the following is not true of small inbreeding populations?

There is an increased incidence of recessive diseases
Mutation is increased
Alleles may become fixed
They tend to loose genetic diversity

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
A human cDNA library

None of these
contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive
contains DNA for virtually all of the human proteins in vectors

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence

ANSWER DOWNLOAD EXAMIANS APP