Recombinant DNA Technology
A human cDNA library

contains DNA for virtually all of the human proteins in vectors
None of these
contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive

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Recombinant DNA Technology
Knockout mice are created by

infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

different length fragments of DNA resulting from loss or gain of a restriction site
an Eco RI cuts DNA at a different sequence than Hind III
All of these
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

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