Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern

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Recombinant DNA Technology
A human cDNA library

contains DNA for virtually all of the human proteins in vectors
cannot be used to obtain human genes because it would be radioactive
contains DNA for specific human proteins
None of these

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Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines

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Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
All of these
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains an origin of replication

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