Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

None of these
Protein-coding genes are always associated with a restriction pattern
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested

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Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive
contains DNA for virtually all of the human proteins in vectors
None of these

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

PCR-amplified variable numbers of tandem repeats (VNTRs)
short sequences that will hybridize only to a specific base sequence
mutagenized copies of a gene
copies of the gene with an altered sequence so that a restriction site is inserted

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