Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Northern blot Southern blot Eastern blot Western blot Northern blot Southern blot Eastern blot Western blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector All of these always contains DNA segments for the regulation of mRNA production usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication All of these always contains DNA segments for the regulation of mRNA production usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. positive to negative basic ... acidic negative ... positive long ... short positive to negative basic ... acidic negative ... positive long ... short ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends restriction fragments single strands ligases sticky ends restriction fragments single strands ligases ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are mutagenized copies of a gene copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
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