Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Eastern blot Western blot Northern blot Southern blot Eastern blot Western blot Northern blot Southern blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Isoschizomers recognize same recognition sequence but different recognition site same recognition site and different recognition sequence same recognition site and recognition sequence different recognition site and different recognition sequence same recognition sequence but different recognition site same recognition site and different recognition sequence same recognition site and recognition sequence different recognition site and different recognition sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type IV Type III Type I Type II Type IV Type III Type I Type II ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are copies of the gene with an altered sequence so that a restriction site is inserted short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector always contains DNA segments for the regulation of mRNA production always contains an origin of replication All of these usually contains a gene that confers antibiotic resistance to the bacterial host always contains DNA segments for the regulation of mRNA production always contains an origin of replication All of these usually contains a gene that confers antibiotic resistance to the bacterial host ANSWER DOWNLOAD EXAMIANS APP
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