Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Northern blot Eastern blot Western blot Southern blot Northern blot Eastern blot Western blot Southern blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have restriction fragments ligases sticky ends single strands restriction fragments ligases sticky ends single strands ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which type of restriction enzymes do not usually require ATP? Type IV Type I Type II Type III Type IV Type I Type II Type III ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication always contains DNA segments for the regulation of mRNA production All of these usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication always contains DNA segments for the regulation of mRNA production All of these ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS