Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Southern blot Northern blot Eastern blot Western blot Southern blot Northern blot Eastern blot Western blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Mbo I & Sau 3A Msp I & HPA II Eco RI & MspI Sau 3A & Bam HI Mbo I & Sau 3A Msp I & HPA II Eco RI & MspI Sau 3A & Bam HI ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. long ... short basic ... acidic positive to negative negative ... positive long ... short basic ... acidic positive to negative negative ... positive ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is not true of small inbreeding populations? Mutation is increased They tend to loose genetic diversity Alleles may become fixed There is an increased incidence of recessive diseases Mutation is increased They tend to loose genetic diversity Alleles may become fixed There is an increased incidence of recessive diseases ANSWER DOWNLOAD EXAMIANS APP
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