Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Southern blot Northern blot Eastern blot Western blot Southern blot Northern blot Eastern blot Western blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of these restriction enzymes produce blunt ends? HindIII EcoRV XhoI SaII HindIII EcoRV XhoI SaII ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Isoschizomers recognize same recognition site and recognition sequence same recognition site and different recognition sequence same recognition sequence but different recognition site different recognition site and different recognition sequence same recognition site and recognition sequence same recognition site and different recognition sequence same recognition sequence but different recognition site different recognition site and different recognition sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site All of these cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA primase DNA ligase DNA polymerase DNA helicase DNA primase DNA ligase DNA polymerase DNA helicase ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS