Recombinant DNA Technology
In order to insert a foreign gene into a plasmid, both must __________

originate from the same type of cell
have identical DNA sequences
be cut by the same restriction enzyme
be of the same length

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Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring

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