Recombinant DNA Technology
In order to insert a foreign gene into a plasmid, both must __________

be of the same length
originate from the same type of cell
have identical DNA sequences
be cut by the same restriction enzyme

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

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Recombinant DNA Technology
Isoschizomers recognize

same recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and recognition sequence
different recognition site and different recognition sequence

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines

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