Recombinant DNA Technology
In order to insert a foreign gene into a plasmid, both must __________

originate from the same type of cell
be cut by the same restriction enzyme
have identical DNA sequences
be of the same length

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

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Recombinant DNA Technology
A human cDNA library

contains DNA for virtually all of the human proteins in vectors
cannot be used to obtain human genes because it would be radioactive
contains DNA for specific human proteins
None of these

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