Recombinant DNA Technology
In order to insert a foreign gene into a plasmid, both must __________

be cut by the same restriction enzyme
be of the same length
originate from the same type of cell
have identical DNA sequences

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

short sequences that will hybridize only to a specific base sequence
PCR-amplified variable numbers of tandem repeats (VNTRs)
mutagenized copies of a gene
copies of the gene with an altered sequence so that a restriction site is inserted

ANSWER DOWNLOAD EXAMIANS APP