Recombinant DNA Technology
An expression vector

usually contains a gene that confers antibiotic resistance to the bacterial host
always contains an origin of replication
always contains DNA segments for the regulation of mRNA production
All of these

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus

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