Recombinant DNA Technology
An expression vector

always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production
All of these

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
All of these
different length fragments of DNA resulting from loss or gain of a restriction site
an Eco RI cuts DNA at a different sequence than Hind III

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