Recombinant DNA Technology
An expression vector

All of these
always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Protein-coding genes are always associated with a restriction pattern
None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

All of these
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
an Eco RI cuts DNA at a different sequence than Hind III
different length fragments of DNA resulting from loss or gain of a restriction site

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