Recombinant DNA Technology An expression vector usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains an origin of replication always contains DNA segments for the regulation of mRNA production usually contains a gene that confers antibiotic resistance to the bacterial host All of these always contains an origin of replication always contains DNA segments for the regulation of mRNA production ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA ligase DNA primase DNA polymerase DNA helicase DNA ligase DNA primase DNA polymerase DNA helicase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene copies of the gene with an altered sequence so that a restriction site is inserted ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Restriction enzymes are named for None of these the viral DNA that they attack the bacterium they are derived from the person who discovered None of these the viral DNA that they attack the bacterium they are derived from the person who discovered ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is not true of small inbreeding populations? They tend to loose genetic diversity Mutation is increased Alleles may become fixed There is an increased incidence of recessive diseases They tend to loose genetic diversity Mutation is increased Alleles may become fixed There is an increased incidence of recessive diseases ANSWER DOWNLOAD EXAMIANS APP
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