Recombinant DNA Technology An expression vector always contains an origin of replication always contains DNA segments for the regulation of mRNA production All of these usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication always contains DNA segments for the regulation of mRNA production All of these usually contains a gene that confers antibiotic resistance to the bacterial host ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of these restriction enzymes produce blunt ends? XhoI SaII EcoRV HindIII XhoI SaII EcoRV HindIII ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is an Eco RI cuts DNA at a different sequence than Hind III All of these different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved an Eco RI cuts DNA at a different sequence than Hind III All of these different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ originate from the same type of cell be of the same length be cut by the same restriction enzyme have identical DNA sequences originate from the same type of cell be of the same length be cut by the same restriction enzyme have identical DNA sequences ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The RP13 gene of chromosome 17 codes for a protein __________ . involved in glucose transport involved in the determination of personality that is a component of hair and nails involved in eye development involved in glucose transport involved in the determination of personality that is a component of hair and nails involved in eye development ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT ANSWER DOWNLOAD EXAMIANS APP
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