Recombinant DNA Technology
An expression vector

always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host
All of these
always contains DNA segments for the regulation of mRNA production

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence

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Recombinant DNA Technology
Isoschizomers recognize

different recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and different recognition sequence
same recognition site and recognition sequence

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

None of these
Protein-coding genes are always associated with a restriction pattern
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested

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