Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have single strands ligases sticky ends restriction fragments single strands ligases sticky ends restriction fragments ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Northern blot Eastern blot Southern blot Western blot Northern blot Eastern blot Southern blot Western blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are copies of the gene with an altered sequence so that a restriction site is inserted short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology First discovered, Type II restriction endonuclease was EcoRI Eco K Hind II Hinf I EcoRI Eco K Hind II Hinf I ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because in both cases molecules migrate to the anode both techniques rely on a constant charge to mass ratio both techniques utilize the sieving properties of gels All of these in both cases molecules migrate to the anode both techniques rely on a constant charge to mass ratio both techniques utilize the sieving properties of gels All of these ANSWER DOWNLOAD EXAMIANS APP
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