Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have single strands restriction fragments sticky ends ligases single strands restriction fragments sticky ends ligases ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following is correct in terms of determination of location of genetic traits? None of these Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes Restriction sites allow DNAs to be digested None of these Protein-coding genes are always associated with a restriction pattern Known protein coding sequences are too far apart to allow linkage determination for most new genes Restriction sites allow DNAs to be digested ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector always contains an origin of replication All of these always contains DNA segments for the regulation of mRNA production usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication All of these always contains DNA segments for the regulation of mRNA production usually contains a gene that confers antibiotic resistance to the bacterial host ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology First discovered, Type II restriction endonuclease was Hinf I EcoRI Eco K Hind II Hinf I EcoRI Eco K Hind II ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene ANSWER DOWNLOAD EXAMIANS APP
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