Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends single strands ligases restriction fragments sticky ends single strands ligases restriction fragments ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Msp I & HPA II Sau 3A & Bam HI Eco RI & MspI Mbo I & Sau 3A Msp I & HPA II Sau 3A & Bam HI Eco RI & MspI Mbo I & Sau 3A ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An expression vector always contains DNA segments for the regulation of mRNA production usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication All of these always contains DNA segments for the regulation of mRNA production usually contains a gene that confers antibiotic resistance to the bacterial host always contains an origin of replication All of these ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus mutagenizing a mouse and selecting for mutant offspring transfecting embryonic stem cells with an altered gene sequence creating a chimera by fusing cells from two different cell lines ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid All of these usually contains one or more restriction sites is a circular DNA molecule always contains an origin of replication All of these usually contains one or more restriction sites is a circular DNA molecule always contains an origin of replication ANSWER DOWNLOAD EXAMIANS APP
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