Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends single strands ligases restriction fragments sticky ends single strands ligases restriction fragments ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because both techniques rely on a constant charge to mass ratio All of these in both cases molecules migrate to the anode both techniques utilize the sieving properties of gels both techniques rely on a constant charge to mass ratio All of these in both cases molecules migrate to the anode both techniques utilize the sieving properties of gels ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following techniques can be used to determine the defective gene and for developing cancer? Western blot Eastern blot Southern blot Northern blot Western blot Eastern blot Southern blot Northern blot ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Msp I & HPA II Sau 3A & Bam HI Mbo I & Sau 3A Eco RI & MspI Msp I & HPA II Sau 3A & Bam HI Mbo I & Sau 3A Eco RI & MspI ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. long ... short positive to negative negative ... positive basic ... acidic long ... short positive to negative negative ... positive basic ... acidic ANSWER DOWNLOAD EXAMIANS APP