Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have sticky ends single strands restriction fragments ligases sticky ends single strands restriction fragments ligases ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ be of the same length originate from the same type of cell have identical DNA sequences be cut by the same restriction enzyme be of the same length originate from the same type of cell have identical DNA sequences be cut by the same restriction enzyme ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid usually contains one or more restriction sites All of these is a circular DNA molecule always contains an origin of replication usually contains one or more restriction sites All of these is a circular DNA molecule always contains an origin of replication ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Msp I & HPA II Sau 3A & Bam HI Mbo I & Sau 3A Eco RI & MspI Msp I & HPA II Sau 3A & Bam HI Mbo I & Sau 3A Eco RI & MspI ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A human cDNA library None of these cannot be used to obtain human genes because it would be radioactive contains DNA for specific human proteins contains DNA for virtually all of the human proteins in vectors None of these cannot be used to obtain human genes because it would be radioactive contains DNA for specific human proteins contains DNA for virtually all of the human proteins in vectors ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
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