Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence
PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

an Eco RI cuts DNA at a different sequence than Hind III
All of these
different length fragments of DNA resulting from loss or gain of a restriction site
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

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Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive
contains DNA for virtually all of the human proteins in vectors
None of these

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