Recombinant DNA Technology
Why is golden rice pale yellow in color?

It is rich in phycobilins.
It is rich in chlorophyll a.
It is rich in chlorophyll b.
It is rich in beta-carotene.

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

short sequences that will hybridize only to a specific base sequence
mutagenized copies of a gene
PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted

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Recombinant DNA Technology
An expression vector

usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production
always contains an origin of replication
All of these

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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