Recombinant DNA Technology A plasmid usually contains one or more restriction sites All of these always contains an origin of replication is a circular DNA molecule usually contains one or more restriction sites All of these always contains an origin of replication is a circular DNA molecule ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA ligase DNA helicase DNA polymerase DNA primase DNA ligase DNA helicase DNA polymerase DNA primase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is All of these an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved All of these an Eco RI cuts DNA at a different sequence than Hind III different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Knockout mice are created by creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring creating a chimera by fusing cells from two different cell lines infecting the mouse with a retrovirus transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Under which of the following conditions would population gene frequencies remain the same? Active migration between groups Random mating Selection for homozygotes Small population size Active migration between groups Random mating Selection for homozygotes Small population size ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The order for the construction of a cDNA fragment from mRNA is to bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase ANSWER DOWNLOAD EXAMIANS APP
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