Recombinant DNA Technology
A plasmid

always contains an origin of replication
is a circular DNA molecule
All of these
usually contains one or more restriction sites

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

PCR-amplified variable numbers of tandem repeats (VNTRs)
copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
different length fragments of DNA resulting from loss or gain of a restriction site
an Eco RI cuts DNA at a different sequence than Hind III
All of these

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