Recombinant DNA Technology
A plasmid

is a circular DNA molecule
always contains an origin of replication
All of these
usually contains one or more restriction sites

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern
Known protein coding sequences are too far apart to allow linkage determination for most new genes
None of these

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