Recombinant DNA Technology
A plasmid

usually contains one or more restriction sites
All of these
always contains an origin of replication
is a circular DNA molecule

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
an Eco RI cuts DNA at a different sequence than Hind III
All of these
different length fragments of DNA resulting from loss or gain of a restriction site

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