Recombinant DNA Technology
Plant transformation can be obtained by

infecting plants with a tobacco mosaic virus
shooting DNA into plant cells with a gun
using the E. coli bacterium to infect plant roots
combining plant and animal cells in culture

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Protein-coding genes are always associated with a restriction pattern
None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested

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