Recombinant DNA Technology
Plant transformation can be obtained by

using the E. coli bacterium to infect plant roots
infecting plants with a tobacco mosaic virus
shooting DNA into plant cells with a gun
combining plant and animal cells in culture

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase

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Recombinant DNA Technology
A human cDNA library

contains DNA for specific human proteins
cannot be used to obtain human genes because it would be radioactive
None of these
contains DNA for virtually all of the human proteins in vectors

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