Recombinant DNA Technology
Plant transformation can be obtained by

combining plant and animal cells in culture
using the E. coli bacterium to infect plant roots
infecting plants with a tobacco mosaic virus
shooting DNA into plant cells with a gun

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host
All of these
always contains DNA segments for the regulation of mRNA production

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