Recombinant DNA Technology
Plant transformation can be obtained by

combining plant and animal cells in culture
infecting plants with a tobacco mosaic virus
using the E. coli bacterium to infect plant roots
shooting DNA into plant cells with a gun

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Known protein coding sequences are too far apart to allow linkage determination for most new genes
None of these
Protein-coding genes are always associated with a restriction pattern
Restriction sites allow DNAs to be digested

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
All of these
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production

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