Recombinant DNA Technology
Plant transformation can be obtained by

shooting DNA into plant cells with a gun
combining plant and animal cells in culture
infecting plants with a tobacco mosaic virus
using the E. coli bacterium to infect plant roots

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern
Known protein coding sequences are too far apart to allow linkage determination for most new genes
None of these

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Recombinant DNA Technology
A human cDNA library

cannot be used to obtain human genes because it would be radioactive
None of these
contains DNA for virtually all of the human proteins in vectors
contains DNA for specific human proteins

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

short sequences that will hybridize only to a specific base sequence
copies of the gene with an altered sequence so that a restriction site is inserted
mutagenized copies of a gene
PCR-amplified variable numbers of tandem repeats (VNTRs)

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