Recombinant DNA Technology
Restriction enzymes

All of these
protect bacteria from viral infection
cut DNAs producing a blunt end
cut DNA in a staggered fashion

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Recombinant DNA Technology
An example of a restriction fragment length polymorphism is

All of these
an Eco RI cuts DNA at a different sequence than Hind III
different length fragments of DNA resulting from loss or gain of a restriction site
cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

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Recombinant DNA Technology
Isoschizomers recognize

same recognition site and different recognition sequence
same recognition sequence but different recognition site
different recognition site and different recognition sequence
same recognition site and recognition sequence

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

copies of the gene with an altered sequence so that a restriction site is inserted
short sequences that will hybridize only to a specific base sequence
mutagenized copies of a gene
PCR-amplified variable numbers of tandem repeats (VNTRs)

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