Recombinant DNA Technology
Restriction enzymes

cut DNA in a staggered fashion
protect bacteria from viral infection
All of these
cut DNAs producing a blunt end

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Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines
mutagenizing a mouse and selecting for mutant offspring

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