Recombinant DNA Technology Restriction enzymes All of these protect bacteria from viral infection cut DNAs producing a blunt end cut DNA in a staggered fashion All of these protect bacteria from viral infection cut DNAs producing a blunt end cut DNA in a staggered fashion ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of the following pair will produce complementary sticky ends? Sau 3A & Bam HI Msp I & HPA II Mbo I & Sau 3A Eco RI & MspI Sau 3A & Bam HI Msp I & HPA II Mbo I & Sau 3A Eco RI & MspI ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology The unpaired nucleotides produced by the action of restriction enzymes are referred to have restriction fragments sticky ends ligases single strands restriction fragments sticky ends ligases single strands ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ be of the same length be cut by the same restriction enzyme have identical DNA sequences originate from the same type of cell be of the same length be cut by the same restriction enzyme have identical DNA sequences originate from the same type of cell ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. negative ... positive long ... short basic ... acidic positive to negative negative ... positive long ... short basic ... acidic positive to negative ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are copies of the gene with an altered sequence so that a restriction site is inserted short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene PCR-amplified variable numbers of tandem repeats (VNTRs) ANSWER DOWNLOAD EXAMIANS APP
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