Recombinant DNA Technology
Restriction enzymes

cut DNAs producing a blunt end
protect bacteria from viral infection
All of these
cut DNA in a staggered fashion

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Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
mutagenizing a mouse and selecting for mutant offspring
infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Restriction sites allow DNAs to be digested
Protein-coding genes are always associated with a restriction pattern
None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes

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