Recombinant DNA Technology
Restriction enzymes

All of these
cut DNA in a staggered fashion
protect bacteria from viral infection
cut DNAs producing a blunt end

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Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus

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Recombinant DNA Technology
A human cDNA library

None of these
contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors
cannot be used to obtain human genes because it would be radioactive

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