Recombinant DNA Technology
Restriction enzymes

protect bacteria from viral infection
cut DNAs producing a blunt end
cut DNA in a staggered fashion
All of these

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

mutagenized copies of a gene
copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)
short sequences that will hybridize only to a specific base sequence

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Recombinant DNA Technology
Isoschizomers recognize

same recognition sequence but different recognition site
same recognition site and different recognition sequence
different recognition site and different recognition sequence
same recognition site and recognition sequence

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