Recombinant DNA Technology
An expression vector

always contains DNA segments for the regulation of mRNA production
All of these
always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host

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Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

mutagenized copies of a gene
copies of the gene with an altered sequence so that a restriction site is inserted
PCR-amplified variable numbers of tandem repeats (VNTRs)
short sequences that will hybridize only to a specific base sequence

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Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring

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