Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

mutagenized copies of a gene
copies of the gene with an altered sequence so that a restriction site is inserted
short sequences that will hybridize only to a specific base sequence
PCR-amplified variable numbers of tandem repeats (VNTRs)

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Restriction sites allow DNAs to be digested
None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Protein-coding genes are always associated with a restriction pattern

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Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

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Recombinant DNA Technology
An expression vector

All of these
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains an origin of replication
always contains DNA segments for the regulation of mRNA production

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