Recombinant DNA Technology
Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

PCR-amplified variable numbers of tandem repeats (VNTRs)
mutagenized copies of a gene
short sequences that will hybridize only to a specific base sequence
copies of the gene with an altered sequence so that a restriction site is inserted

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
The order for the construction of a cDNA fragment from mRNA is to

bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

ANSWER DOWNLOAD EXAMIANS APP

Recombinant DNA Technology
An expression vector

usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production
All of these
always contains an origin of replication

ANSWER DOWNLOAD EXAMIANS APP
MORE MCQ ON Recombinant DNA Technology