Recombinant DNA Technology Knockout mice are created by mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In order to insert a foreign gene into a plasmid, both must __________ be of the same length originate from the same type of cell be cut by the same restriction enzyme have identical DNA sequences be of the same length originate from the same type of cell be cut by the same restriction enzyme have identical DNA sequences ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology A plasmid usually contains one or more restriction sites All of these always contains an origin of replication is a circular DNA molecule usually contains one or more restriction sites All of these always contains an origin of replication is a circular DNA molecule ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology What enzyme forms covalent bonds between restriction fragments? DNA ligase DNA helicase DNA polymerase DNA primase DNA ligase DNA helicase DNA polymerase DNA primase ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence PCR-amplified variable numbers of tandem repeats (VNTRs) copies of the gene with an altered sequence so that a restriction site is inserted mutagenized copies of a gene short sequences that will hybridize only to a specific base sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved an Eco RI cuts DNA at a different sequence than Hind III All of these different length fragments of DNA resulting from loss or gain of a restriction site cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved an Eco RI cuts DNA at a different sequence than Hind III All of these ANSWER DOWNLOAD EXAMIANS APP
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