Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring
transfecting embryonic stem cells with an altered gene sequence

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Recombinant DNA Technology
An expression vector

All of these
always contains DNA segments for the regulation of mRNA production
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains an origin of replication

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Recombinant DNA Technology
A human cDNA library

None of these
cannot be used to obtain human genes because it would be radioactive
contains DNA for virtually all of the human proteins in vectors
contains DNA for specific human proteins

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