Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
transfecting embryonic stem cells with an altered gene sequence
infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring

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Recombinant DNA Technology
A human cDNA library

cannot be used to obtain human genes because it would be radioactive
contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors
None of these

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