Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring
infecting the mouse with a retrovirus
creating a chimera by fusing cells from two different cell lines

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
All of these
usually contains a gene that confers antibiotic resistance to the bacterial host
always contains DNA segments for the regulation of mRNA production

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Protein-coding genes are always associated with a restriction pattern
None of these
Known protein coding sequences are too far apart to allow linkage determination for most new genes
Restriction sites allow DNAs to be digested

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