Recombinant DNA Technology
Knockout mice are created by

transfecting embryonic stem cells with an altered gene sequence
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
mutagenizing a mouse and selecting for mutant offspring

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Recombinant DNA Technology
Which of the following is correct in terms of determination of location of genetic traits?

Known protein coding sequences are too far apart to allow linkage determination for most new genes
Protein-coding genes are always associated with a restriction pattern
Restriction sites allow DNAs to be digested
None of these

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