Recombinant DNA Technology
Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring
creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence

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Recombinant DNA Technology
Isoschizomers recognize

same recognition site and different recognition sequence
same recognition sequence but different recognition site
same recognition site and recognition sequence
different recognition site and different recognition sequence

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Recombinant DNA Technology
An expression vector

All of these
always contains DNA segments for the regulation of mRNA production
always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host

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Recombinant DNA Technology
A human cDNA library

contains DNA for virtually all of the human proteins in vectors
contains DNA for specific human proteins
None of these
cannot be used to obtain human genes because it would be radioactive

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