Recombinant DNA Technology
Knockout mice are created by

creating a chimera by fusing cells from two different cell lines
infecting the mouse with a retrovirus
transfecting embryonic stem cells with an altered gene sequence
mutagenizing a mouse and selecting for mutant offspring

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Recombinant DNA Technology
An expression vector

always contains an origin of replication
usually contains a gene that confers antibiotic resistance to the bacterial host
All of these
always contains DNA segments for the regulation of mRNA production

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Recombinant DNA Technology
A human cDNA library

cannot be used to obtain human genes because it would be radioactive
None of these
contains DNA for specific human proteins
contains DNA for virtually all of the human proteins in vectors

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