Recombinant DNA Technology Knockout mice are created by creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus creating a chimera by fusing cells from two different cell lines transfecting embryonic stem cells with an altered gene sequence mutagenizing a mouse and selecting for mutant offspring infecting the mouse with a retrovirus ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology First discovered, Type II restriction endonuclease was EcoRI Hinf I Eco K Hind II EcoRI Hinf I Eco K Hind II ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel. positive to negative negative ... positive long ... short basic ... acidic positive to negative negative ... positive long ... short basic ... acidic ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are mutagenized copies of a gene copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence mutagenized copies of a gene copies of the gene with an altered sequence so that a restriction site is inserted PCR-amplified variable numbers of tandem repeats (VNTRs) short sequences that will hybridize only to a specific base sequence ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology Which of these genes codes for a protein that plays a role in white blood cell function? GLUT4 RP13 DCP1 MPO GLUT4 RP13 DCP1 MPO ANSWER DOWNLOAD EXAMIANS APP
Recombinant DNA Technology An example of a restriction fragment length polymorphism is an Eco RI cuts DNA at a different sequence than Hind III cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved different length fragments of DNA resulting from loss or gain of a restriction site All of these an Eco RI cuts DNA at a different sequence than Hind III cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved different length fragments of DNA resulting from loss or gain of a restriction site All of these ANSWER DOWNLOAD EXAMIANS APP