Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A will still be produced, but not B, C, or D
A, B, and C will still be produced, but not D
A and B will still be produced, but not C or D
A, B, C, and D will all still be produced

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket
absence of water in the active site
size, shape, and charge of the active site or specificity pocket

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Enzymes
Before they can react, many molecules need to be destabilized. This state is typically achieved through

the input of a small amount of activation energy
oxidizing the molecules by removing electrons
changing the three-dimensional shape of the molecule
changing the reaction from a biosynthetic to a catabolic pathway

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