Enzymes The proteolysis rate enhancement by chymotrypsin (~1010 folds) corresponds to a reduction in activation energy of about 40 kJ/mol 58 kJ/mol 88 kJ/mol 49 kJ/mol 40 kJ/mol 58 kJ/mol 88 kJ/mol 49 kJ/mol ANSWER DOWNLOAD EXAMIANS APP
Enzymes The role of Asp 102 and His 57 during trypsin catalysis is to keep the specificity pocket open clamp the substrate into the active site neutralize the charge on the other's side chain function as a proton shuttle keep the specificity pocket open clamp the substrate into the active site neutralize the charge on the other's side chain function as a proton shuttle ANSWER DOWNLOAD EXAMIANS APP
Enzymes In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)---> A will still be produced, but not B, C, or D A, B, C, and D will all still be produced A and B will still be produced, but not C or D A, B, and C will still be produced, but not D A will still be produced, but not B, C, or D A, B, C, and D will all still be produced A and B will still be produced, but not C or D A, B, and C will still be produced, but not D ANSWER DOWNLOAD EXAMIANS APP
Enzymes Which of the common features are shared between serine and aspartate proteases? All of these Both show specificity for certain amino acid sequences Both require water to complete the catalytic cycle Both use a base to activate the nucleophile All of these Both show specificity for certain amino acid sequences Both require water to complete the catalytic cycle Both use a base to activate the nucleophile ANSWER DOWNLOAD EXAMIANS APP
Enzymes The cleavage specificity of trypsin and chymotrypsin depend in part on the size, shape, and charge of the active site or specificity pocket absence of water in the active site presence of a low-barrier hydrogen bond in the active site or specificity pocket proximity of Ser 195 to the active site or specificity pocket size, shape, and charge of the active site or specificity pocket absence of water in the active site presence of a low-barrier hydrogen bond in the active site or specificity pocket proximity of Ser 195 to the active site or specificity pocket ANSWER DOWNLOAD EXAMIANS APP
Enzymes What is the specificity of the Clostripain protease? None of these It cleave after His residues It cleave after Lys residues It cleave after Arg residues None of these It cleave after His residues It cleave after Lys residues It cleave after Arg residues ANSWER DOWNLOAD EXAMIANS APP
EXAMIANS