Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

keep the specificity pocket open
clamp the substrate into the active site
neutralize the charge on the other's side chain
function as a proton shuttle

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Enzymes
The E.coli pyruvic acid dehydrogenase complex is reported to

Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2
decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2
retard the reduction of pyruvic acid to acetyl Co A and CO2
Catalyze the reduction of pyruvic acid to acetyl Co A and CO2

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

All of these
concentrating enzymes within specific cellular compartments
grouping enzymes into free-floating, multienzyme complexes
fixing enzymes into membranes so that they are adjacent to each other

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