Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

keep the specificity pocket open
function as a proton shuttle
neutralize the charge on the other's side chain
clamp the substrate into the active site

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Enzymes
Before they can react, many molecules need to be destabilized. This state is typically achieved through

changing the three-dimensional shape of the molecule
changing the reaction from a biosynthetic to a catabolic pathway
the input of a small amount of activation energy
oxidizing the molecules by removing electrons

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

absence of water in the active site
size, shape, and charge of the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket
proximity of Ser 195 to the active site or specificity pocket

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Enzymes
The E.coli pyruvic acid dehydrogenase complex is reported to

decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2
retard the reduction of pyruvic acid to acetyl Co A and CO2
Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2
Catalyze the reduction of pyruvic acid to acetyl Co A and CO2

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

All of these
fixing enzymes into membranes so that they are adjacent to each other
grouping enzymes into free-floating, multienzyme complexes
concentrating enzymes within specific cellular compartments

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