Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

clamp the substrate into the active site
keep the specificity pocket open
function as a proton shuttle
neutralize the charge on the other's side chain

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Enzymes
Before they can react, many molecules need to be destabilized. This state is typically achieved through

oxidizing the molecules by removing electrons
changing the three-dimensional shape of the molecule
changing the reaction from a biosynthetic to a catabolic pathway
the input of a small amount of activation energy

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

presence of a low-barrier hydrogen bond in the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket
absence of water in the active site
proximity of Ser 195 to the active site or specificity pocket

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