Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

clamp the substrate into the active site
keep the specificity pocket open
neutralize the charge on the other's side chain
function as a proton shuttle

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

presence of a low-barrier hydrogen bond in the active site or specificity pocket
proximity of Ser 195 to the active site or specificity pocket
absence of water in the active site
size, shape, and charge of the active site or specificity pocket

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