Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

clamp the substrate into the active site
keep the specificity pocket open
function as a proton shuttle
neutralize the charge on the other's side chain

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
absence of water in the active site
size, shape, and charge of the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

concentrating enzymes within specific cellular compartments
All of these
grouping enzymes into free-floating, multienzyme complexes
fixing enzymes into membranes so that they are adjacent to each other

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