Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

function as a proton shuttle
neutralize the charge on the other's side chain
keep the specificity pocket open
clamp the substrate into the active site

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

concentrating enzymes within specific cellular compartments
All of these
grouping enzymes into free-floating, multienzyme complexes
fixing enzymes into membranes so that they are adjacent to each other

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

absence of water in the active site
size, shape, and charge of the active site or specificity pocket
proximity of Ser 195 to the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket

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Enzymes
Which of the following is false statement with regard to comparison between Serine and HIV proteases?

Both require water to complete the catalytic cycle
Both show specificity for certain amino acid sequences
Both use nucleophilic attack to hydrolyze the peptide bond
Both forms an acyl-enzyme intermediate

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Enzymes
Before they can react, many molecules need to be destabilized. This state is typically achieved through

the input of a small amount of activation energy
oxidizing the molecules by removing electrons
changing the reaction from a biosynthetic to a catabolic pathway
changing the three-dimensional shape of the molecule

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