Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

keep the specificity pocket open
clamp the substrate into the active site
neutralize the charge on the other's side chain
function as a proton shuttle

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

absence of water in the active site
proximity of Ser 195 to the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A will still be produced, but not B, C, or D
A and B will still be produced, but not C or D
A, B, C, and D will all still be produced
A, B, and C will still be produced, but not D

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