Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

keep the specificity pocket open
function as a proton shuttle
clamp the substrate into the active site
neutralize the charge on the other's side chain

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A will still be produced, but not B, C, or D
A, B, and C will still be produced, but not D
A, B, C, and D will all still be produced
A and B will still be produced, but not C or D

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket
absence of water in the active site

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Enzymes
Which of the following statements about enzymes or their function is true?

All of these
Enzymes speed up reactions by lowering activation energy
Enzymes do not alter the overall change in free energy for a reaction
Enzymes are proteins whose three-dimensional form is key to their function

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