Enzymes The role of Asp 102 and His 57 during trypsin catalysis is to clamp the substrate into the active site keep the specificity pocket open function as a proton shuttle neutralize the charge on the other's side chain clamp the substrate into the active site keep the specificity pocket open function as a proton shuttle neutralize the charge on the other's side chain ANSWER DOWNLOAD EXAMIANS APP
Enzymes The nucleophile in serine proteases is Serine both (a) and (b) water Asparagine Serine both (a) and (b) water Asparagine ANSWER DOWNLOAD EXAMIANS APP
Enzymes Before they can react, many molecules need to be destabilized. This state is typically achieved through oxidizing the molecules by removing electrons changing the three-dimensional shape of the molecule changing the reaction from a biosynthetic to a catabolic pathway the input of a small amount of activation energy oxidizing the molecules by removing electrons changing the three-dimensional shape of the molecule changing the reaction from a biosynthetic to a catabolic pathway the input of a small amount of activation energy ANSWER DOWNLOAD EXAMIANS APP
Enzymes What is the specificity of the Clostripain protease? It cleave after His residues It cleave after Arg residues None of these It cleave after Lys residues It cleave after His residues It cleave after Arg residues None of these It cleave after Lys residues ANSWER DOWNLOAD EXAMIANS APP
Enzymes The proteolysis rate enhancement by chymotrypsin (~1010 folds) corresponds to a reduction in activation energy of about 40 kJ/mol 88 kJ/mol 49 kJ/mol 58 kJ/mol 40 kJ/mol 88 kJ/mol 49 kJ/mol 58 kJ/mol ANSWER DOWNLOAD EXAMIANS APP
Enzymes The cleavage specificity of trypsin and chymotrypsin depend in part on the presence of a low-barrier hydrogen bond in the active site or specificity pocket size, shape, and charge of the active site or specificity pocket absence of water in the active site proximity of Ser 195 to the active site or specificity pocket presence of a low-barrier hydrogen bond in the active site or specificity pocket size, shape, and charge of the active site or specificity pocket absence of water in the active site proximity of Ser 195 to the active site or specificity pocket ANSWER DOWNLOAD EXAMIANS APP