Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket
absence of water in the active site

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

grouping enzymes into free-floating, multienzyme complexes
fixing enzymes into membranes so that they are adjacent to each other
concentrating enzymes within specific cellular compartments
All of these

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Enzymes
Before they can react, many molecules need to be destabilized. This state is typically achieved through

changing the reaction from a biosynthetic to a catabolic pathway
the input of a small amount of activation energy
oxidizing the molecules by removing electrons
changing the three-dimensional shape of the molecule

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