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Enzymes

Enzymes
The nucleophile in serine proteases is

Serine
Asparagine
both (a) and (b)
water

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Enzymes
Which of the common features are shared between serine and aspartate proteases?

Both use a base to activate the nucleophile
Both require water to complete the catalytic cycle
Both show specificity for certain amino acid sequences
All of these

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Enzymes
The role of Asp 102 and His 57 during trypsin catalysis is to

neutralize the charge on the other's side chain
function as a proton shuttle
keep the specificity pocket open
clamp the substrate into the active site

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Enzymes
What is the specificity of the Clostripain protease?

It cleave after Arg residues
None of these
It cleave after His residues
It cleave after Lys residues

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Enzymes
The proteolysis rate enhancement by chymotrypsin (~1010 folds) corresponds to a reduction in activation energy of about

58 kJ/mol
40 kJ/mol
49 kJ/mol
88 kJ/mol

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Enzymes
Tryprotophan synthetase of E.coli, a typical bifunctional oligomeric enzyme consist of

a protein A and one-subunit a
a protein designated A
a protein designated B
two proteins designated A and B

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