Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A will still be produced, but not B, C, or D
A and B will still be produced, but not C or D
A, B, C, and D will all still be produced
A, B, and C will still be produced, but not D

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

presence of a low-barrier hydrogen bond in the active site or specificity pocket
proximity of Ser 195 to the active site or specificity pocket
absence of water in the active site
size, shape, and charge of the active site or specificity pocket

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