Enzymes
What is the specificity of the Clostripain protease?

It cleave after Lys residues
It cleave after His residues
None of these
It cleave after Arg residues

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Enzymes
The E.coli pyruvic acid dehydrogenase complex is reported to

retard the reduction of pyruvic acid to acetyl Co A and CO2
decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2
Catalyze the reduction of pyruvic acid to acetyl Co A and CO2
Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

presence of a low-barrier hydrogen bond in the active site or specificity pocket
absence of water in the active site
size, shape, and charge of the active site or specificity pocket
proximity of Ser 195 to the active site or specificity pocket

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Enzymes
Which of the following is false statement with regard to comparison between Serine and HIV proteases?

Both forms an acyl-enzyme intermediate
Both show specificity for certain amino acid sequences
Both require water to complete the catalytic cycle
Both use nucleophilic attack to hydrolyze the peptide bond

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A, B, and C will still be produced, but not D
A, B, C, and D will all still be produced
A and B will still be produced, but not C or D
A will still be produced, but not B, C, or D

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