Enzymes
What is the specificity of the Clostripain protease?

It cleave after Lys residues
It cleave after His residues
It cleave after Arg residues
None of these

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
absence of water in the active site
presence of a low-barrier hydrogen bond in the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A and B will still be produced, but not C or D
A, B, C, and D will all still be produced
A will still be produced, but not B, C, or D
A, B, and C will still be produced, but not D

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Enzymes
Which of the following is false statement with regard to comparison between Serine and HIV proteases?

Both use nucleophilic attack to hydrolyze the peptide bond
Both forms an acyl-enzyme intermediate
Both show specificity for certain amino acid sequences
Both require water to complete the catalytic cycle

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Enzymes
Enzyme-driven metabolic pathways can be made more efficient by

fixing enzymes into membranes so that they are adjacent to each other
concentrating enzymes within specific cellular compartments
grouping enzymes into free-floating, multienzyme complexes
All of these

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