Enzymes
Which of the common features are shared between serine and aspartate proteases?

All of these
Both show specificity for certain amino acid sequences
Both use a base to activate the nucleophile
Both require water to complete the catalytic cycle

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Enzymes
How is the enzyme COX-1 important in human health?

It is a chemical derivative of aspirin
It is critical for the biosynthesis of DNA
It catalyzes the production of hormones that maintain the stomach lining
It helps to transport carbon dioxide in the blood

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Enzymes
The E.coli pyruvic acid dehydrogenase complex is reported to

Catalyze the reduction of pyruvic acid to acetyl Co A and CO2
decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2
retard the reduction of pyruvic acid to acetyl Co A and CO2
Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A, B, and C will still be produced, but not D
A will still be produced, but not B, C, or D
A and B will still be produced, but not C or D
A, B, C, and D will all still be produced

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

absence of water in the active site
proximity of Ser 195 to the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket

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