Enzymes Which of the common features are shared between serine and aspartate proteases? Both require water to complete the catalytic cycle Both use a base to activate the nucleophile All of these Both show specificity for certain amino acid sequences Both require water to complete the catalytic cycle Both use a base to activate the nucleophile All of these Both show specificity for certain amino acid sequences ANSWER DOWNLOAD EXAMIANS APP
Enzymes The cleavage specificity of trypsin and chymotrypsin depend in part on the absence of water in the active site presence of a low-barrier hydrogen bond in the active site or specificity pocket size, shape, and charge of the active site or specificity pocket proximity of Ser 195 to the active site or specificity pocket absence of water in the active site presence of a low-barrier hydrogen bond in the active site or specificity pocket size, shape, and charge of the active site or specificity pocket proximity of Ser 195 to the active site or specificity pocket ANSWER DOWNLOAD EXAMIANS APP
Enzymes The proteolysis rate enhancement by chymotrypsin (~1010 folds) corresponds to a reduction in activation energy of about 40 kJ/mol 49 kJ/mol 88 kJ/mol 58 kJ/mol 40 kJ/mol 49 kJ/mol 88 kJ/mol 58 kJ/mol ANSWER DOWNLOAD EXAMIANS APP
Enzymes The E.coli pyruvic acid dehydrogenase complex is reported to decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2 retard the reduction of pyruvic acid to acetyl Co A and CO2 Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2 Catalyze the reduction of pyruvic acid to acetyl Co A and CO2 decatalyze the oxidation of pyruvic acid to acetyl Co A and CO2 retard the reduction of pyruvic acid to acetyl Co A and CO2 Catalyze the oxidation of pyruvic acid to acetyl Co A and CO2 Catalyze the reduction of pyruvic acid to acetyl Co A and CO2 ANSWER DOWNLOAD EXAMIANS APP
Enzymes The role of Asp 102 and His 57 during trypsin catalysis is to clamp the substrate into the active site keep the specificity pocket open neutralize the charge on the other's side chain function as a proton shuttle clamp the substrate into the active site keep the specificity pocket open neutralize the charge on the other's side chain function as a proton shuttle ANSWER DOWNLOAD EXAMIANS APP
Enzymes Common feature in all serine proteases is a hydrophobic specificity pocket single reactive serine residue hydrophilic specificity pocket cluster of reactive serine residues hydrophobic specificity pocket single reactive serine residue hydrophilic specificity pocket cluster of reactive serine residues ANSWER DOWNLOAD EXAMIANS APP
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