Enzymes
Which of the common features are shared between serine and aspartate proteases?

Both use a base to activate the nucleophile
Both require water to complete the catalytic cycle
All of these
Both show specificity for certain amino acid sequences

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Enzymes
In the enzyme-catalyzed reaction shown below, what will be the effect on substances A, B, C, and D of inactivating the enzyme labeled E2? A ---(E1)---> B ---(E2)---> C ---(E3)--->

A will still be produced, but not B, C, or D
A, B, C, and D will all still be produced
A, B, and C will still be produced, but not D
A and B will still be produced, but not C or D

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Enzymes
The cleavage specificity of trypsin and chymotrypsin depend in part on the

proximity of Ser 195 to the active site or specificity pocket
size, shape, and charge of the active site or specificity pocket
presence of a low-barrier hydrogen bond in the active site or specificity pocket
absence of water in the active site

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