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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following histone is not present in SV40 virus?

H1
H4
H3
H2A

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Vectors Uses for Animal Cell Culture
Which one of the following is the most stable vector?

YEp
YRp
YIp
YAC

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Allows transformation by using crossing over
Carries centromere and telomere sequences
Carries autonomously replicating sequence
Has a origin of replication and rep genes

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

SV 40 helper virus
none of these
COS cell line
pBR 322 vector

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

integrated only
either episomally or integrated at high copy number
either episomally or integrated at low copy number
episomally only

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

essential for baculo virus replication
non essential for baculo virus replication
major component of protein matrix in which viruses are embedded
both (a) and (b)

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