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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which one of the following is the most stable vector?

YEp
YRp
YIp
YAC

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Over production of proteins of commercial value
Ability to glycosylate proteins during secretion
Ability to clone large piece of DNA
All of these

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Vectors Uses for Animal Cell Culture
α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1
Ura 3
His 3
Lys 2

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

pBR 322 vector
SV 40 helper virus
none of these
COS cell line

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only
integrated only
either episomally or integrated at high copy number
either episomally or integrated at low copy number

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Vectors Uses for Animal Cell Culture
The size of the genome of SV 40 is

5.2 kb
7.2 kb
69 kb
110 kb

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