Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries centromere and telomere sequences
Carries autonomously replicating sequence
Allows transformation by using crossing over
Has a origin of replication and rep genes

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Vectors Uses for Animal Cell Culture
Retroviruses have advantage for being used as vector for animal cells because

viral gene expression is driven by strong promoters
All of these
infection does not lead to cell death, infected cells produce virus over an indefinite period
they cover a wide host range

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

not necessary for viral replication
important for packaging of viral DNA into viral head
transcriptional regulators and are essential for viral replication
coding for virus coat protein

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