Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Carries centromere and telomere sequences
Allows transformation by using crossing over
Has a origin of replication and rep genes
Carries autonomously replicating sequence

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Vectors Uses for Animal Cell Culture
Retroviruses have advantage for being used as vector for animal cells because

they cover a wide host range
viral gene expression is driven by strong promoters
All of these
infection does not lead to cell death, infected cells produce virus over an indefinite period

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Vectors Uses for Animal Cell Culture
Yeast episomal plasmids (YEp) were constructed using

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
12μm yeast plasmid and E coli vector pBR 322
12μm yeast plasmid and E coli vector pMB 9
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9

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