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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1
Ura 3
His 3
Lys 2

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

none of these
pBR 322 vector
SV 40 helper virus
COS cell line

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Vectors Uses for Animal Cell Culture
Cos cell line is

cohesive end site of phage λ
derivative of non-permissive CV-1 monkey cell line
derivative of permissive CV-1 monkey cell line
cohesive initial site of phage λ

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

major component of protein matrix in which viruses are embedded
essential for baculo virus replication
both (a) and (b)
non essential for baculo virus replication

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Vectors Uses for Animal Cell Culture
Lytic infection of SV 40 does not occur in

none of these
permissive cells
both (a) and (b)
non permissive cells

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at high copy number
episomally only
either episomally or integrated at low copy number
integrated only

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