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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3
Trp 1
Lys 2
His 3

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Ability to glycosylate proteins during secretion
All of these
Ability to clone large piece of DNA
Over production of proteins of commercial value

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Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

5 kb
1 kb
2.5 kb
10 kb

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Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Phosphoglycerate kinase (PGK)
Alcohol dehydrogenase II (ADH 2)
All of these
Galactokinase (GAL 1)

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Vectors Uses for Animal Cell Culture
Which of the following promoters is repressed in presence of glucose?

Galactokinase (GAL 1)
Both (a) and (b)
Phosphoglycerate kinase (PGK)
Acid phosphatase (PHOS)

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Vectors Uses for Animal Cell Culture
Lytic infection of SV 40 does not occur in

non permissive cells
permissive cells
both (a) and (b)
none of these

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