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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3
Trp 1
His 3
Lys 2

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Vectors Uses for Animal Cell Culture
Recombinant SV40 viruses introduce the foreign DNA into cells

with the RNA mediated transfection
with the DNA mediated transfection
without the RNA mediated transfection
without the DNA mediated transfection

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

none of these
SV 40 helper virus
COS cell line
pBR 322 vector

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Vectors Uses for Animal Cell Culture
Which of the following can be used for reducing the size of gene inserts containing introns?

none of these
Retro virus
Vaccinia virus
Baculo virus

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries autonomously replicating sequence
Has a origin of replication and rep genes
Allows transformation by using crossing over
Carries centromere and telomere sequences

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Vectors Uses for Animal Cell Culture
Which of the following(s) is/are the most commonly used markers in yeast?

All of these
His 3
Ura 3
Trp 1

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