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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

All of these
Galactokinase (GAL 1)
Alcohol dehydrogenase II (ADH 2)
Phosphoglycerate kinase (PGK)

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Vectors Uses for Animal Cell Culture
α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1
Ura 3
Lys 2
His 3

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

none of these
SV 40 helper virus
COS cell line
pBR 322 vector

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Vectors Uses for Animal Cell Culture
YAC vectors can be used to obtain artificial chromosomes because it carries

autonomously replicating sequence
telomere
all of these
centromere

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only
integrated only
either episomally or integrated at high copy number
either episomally or integrated at low copy number

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Has a origin of replication and rep genes
Allows transformation by using crossing over
Carries autonomously replicating sequence
Carries centromere and telomere sequences

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