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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Alcohol dehydrogenase II (ADH 2)
All of these
Phosphoglycerate kinase (PGK)
Galactokinase (GAL 1)

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Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

telomere
autonomously replicating sequence
centromere
both (a) and (c)

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Over production of proteins of commercial value
All of these
Ability to glycosylate proteins during secretion
Ability to clone large piece of DNA

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

SV 40 helper virus
COS cell line
none of these
pBR 322 vector

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Vectors Uses for Animal Cell Culture
α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

His 3
Trp 1
Lys 2
Ura 3

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Has a origin of replication and rep genes
Allows transformation by using crossing over
Carries centromere and telomere sequences
Carries autonomously replicating sequence

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