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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Alcohol dehydrogenase II (ADH 2)
All of these
Galactokinase (GAL 1)
Phosphoglycerate kinase (PGK)

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

none of these
pBR 322 vector
SV 40 helper virus
COS cell line

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Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

10 kb
2.5 kb
5 kb
1 kb

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at low copy number
either episomally or integrated at high copy number
integrated only
episomally only

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Vectors Uses for Animal Cell Culture
Lytic infection of SV 40 does not occur in

none of these
permissive cells
non permissive cells
both (a) and (b)

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Vectors Uses for Animal Cell Culture
Which of the following promoters is repressed in presence of glucose?

Both (a) and (b)
Phosphoglycerate kinase (PGK)
Acid phosphatase (PHOS)
Galactokinase (GAL 1)

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