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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Alcohol dehydrogenase II (ADH 2)
Phosphoglycerate kinase (PGK)
Galactokinase (GAL 1)
All of these

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

His 3
Ura 3
Trp 1
Lys 2

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

pBR 322 vector
none of these
COS cell line
SV 40 helper virus

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Carries autonomously replicating sequence
Carries centromere and telomere sequences
Allows transformation by using crossing over
Has a origin of replication and rep genes

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Vectors Uses for Animal Cell Culture
Autonomously replicating sequence is not present in

YIp
YAC
YRp
YCp

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Vectors Uses for Animal Cell Culture
YAC vectors can be used to obtain artificial chromosomes because it carries

centromere
all of these
autonomously replicating sequence
telomere

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