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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Galactokinase (GAL 1)
Alcohol dehydrogenase II (ADH 2)
All of these
Phosphoglycerate kinase (PGK)

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only
either episomally or integrated at high copy number
either episomally or integrated at low copy number
integrated only

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Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

telomere
centromere
autonomously replicating sequence
both (a) and (c)

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Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

YEp
Both (a) and (b)
YCp
YRp

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3
Trp 1
Lys 2
His 3

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Vectors Uses for Animal Cell Culture
Moloney-Mu-leukemia virus is an example of

none of these
Retro virus
Baculo virus
Vaccinia virus

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