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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Alcohol dehydrogenase II (ADH 2)
Phosphoglycerate kinase (PGK)
Galactokinase (GAL 1)
All of these

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Allows transformation by using crossing over
Carries centromere and telomere sequences
Carries autonomously replicating sequence
Has a origin of replication and rep genes

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

pBR 322 vector
none of these
SV 40 helper virus
COS cell line

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Segregates during meiosis and mitosis
All of these
Functions as true chromosome
Contains centromeric sequences

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Vectors Uses for Animal Cell Culture
Autonomously replicating sequence is not present in

YCp
YAC
YRp
YIp

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Vectors Uses for Animal Cell Culture
Which of the following(s) is/are the most commonly used markers in yeast?

Trp 1
His 3
Ura 3
All of these

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