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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Galactokinase (GAL 1)
All of these
Alcohol dehydrogenase II (ADH 2)
Phosphoglycerate kinase (PGK)

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Vectors Uses for Animal Cell Culture
α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Trp 1
Ura 3
Lys 2
His 3

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Vectors Uses for Animal Cell Culture
Which of the following(s) is/are the most commonly used markers in yeast?

All of these
His 3
Ura 3
Trp 1

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

SV 40 helper virus
none of these
pBR 322 vector
COS cell line

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Has a origin of replication and rep genes
Carries autonomously replicating sequence
Carries centromere and telomere sequences
Allows transformation by using crossing over

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

essential for baculo virus replication
major component of protein matrix in which viruses are embedded
non essential for baculo virus replication
both (a) and (b)

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