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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

All of these
Phosphoglycerate kinase (PGK)
Galactokinase (GAL 1)
Alcohol dehydrogenase II (ADH 2)

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Vectors Uses for Animal Cell Culture
Lytic infection of SV 40 does not occur in

permissive cells
non permissive cells
none of these
both (a) and (b)

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Vectors Uses for Animal Cell Culture
Which one of the following is the most stable vector?

YIp
YRp
YAC
YEp

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Vectors Uses for Animal Cell Culture
Autonomously replicating sequence is not present in

YCp
YIp
YRp
YAC

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Functions as true chromosome
All of these
Segregates during meiosis and mitosis
Contains centromeric sequences

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Has a origin of replication and rep genes
Carries autonomously replicating sequence
Carries centromere and telomere sequences
Allows transformation by using crossing over

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