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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Phosphoglycerate kinase (PGK)
Galactokinase (GAL 1)
Alcohol dehydrogenase II (ADH 2)
All of these

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

All of these
Over production of proteins of commercial value
Ability to clone large piece of DNA
Ability to glycosylate proteins during secretion

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Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

YCp
YRp
Both (a) and (b)
YEp

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Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

10 kb
2.5 kb
1 kb
5 kb

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only
integrated only
either episomally or integrated at high copy number
either episomally or integrated at low copy number

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Vectors Uses for Animal Cell Culture
Which of the following viruses are used as vectors for transferring genes to animal cells?

Bovine papilloma virus (BVP)
All of these
SV 40 virus
Retroviruses

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