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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following promoters is repressed in presence of glucose?

Galactokinase (GAL 1)
Both (a) and (b)
Phosphoglycerate kinase (PGK)
Acid phosphatase (PHOS)

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries autonomously replicating sequence
Has a origin of replication and rep genes
Carries centromere and telomere sequences
Allows transformation by using crossing over

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only
either episomally or integrated at high copy number
integrated only
either episomally or integrated at low copy number

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Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

1 kb
5 kb
10 kb
2.5 kb

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Vectors Uses for Animal Cell Culture
Bovine papilloma virus has a circular double stranded DNA genome of about

9.5 kb
7.9 kb
2.5 kb
5.2 kb

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Centromeric plasmids (YCp) vectors?

Functions as true chromosome
Segregates during meiosis and mitosis
Contains centromeric sequences
All of these

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