Vectors Uses for Animal Cell Culture
In case of Baculoviruses, the foreign gene

is inserted randomly in viral genome
is inserted downstream of viral origin of replication
is inserted between the polyhedrin promoter and downstream sequences
is inserted downstream of polyhedrin promoter

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Vectors Uses for Animal Cell Culture
Retroviruses have advantage for being used as vector for animal cells because

All of these
they cover a wide host range
infection does not lead to cell death, infected cells produce virus over an indefinite period
viral gene expression is driven by strong promoters

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

transcriptional regulators and are essential for viral replication
not necessary for viral replication
important for packaging of viral DNA into viral head
coding for virus coat protein

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