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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following viruses are used as vectors for transferring genes to animal cells?

Retroviruses
SV 40 virus
All of these
Bovine papilloma virus (BVP)

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Vectors Uses for Animal Cell Culture
Maximum insert size in case of adenovirus vectors is

20 kb
4-6 kb
6-8 kb
10-12 kb

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which early region replacements are carried out, require

none of these
COS cell line
pBR 322 vector
SV 40 helper virus

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at high copy number
episomally only
integrated only
either episomally or integrated at low copy number

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Vectors Uses for Animal Cell Culture
Recombinant SV40 viruses introduce the foreign DNA into cells

without the DNA mediated transfection
with the DNA mediated transfection
without the RNA mediated transfection
with the RNA mediated transfection

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Vectors Uses for Animal Cell Culture
YAC vectors can be used to obtain artificial chromosomes because it carries

centromere
all of these
telomere
autonomously replicating sequence

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