Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

not necessary for viral replication
coding for virus coat protein
transcriptional regulators and are essential for viral replication
important for packaging of viral DNA into viral head

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Vectors Uses for Animal Cell Culture
In case of Baculoviruses, the foreign gene

is inserted downstream of viral origin of replication
is inserted downstream of polyhedrin promoter
is inserted between the polyhedrin promoter and downstream sequences
is inserted randomly in viral genome

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