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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Bovine papilloma virus has a circular double stranded DNA genome of about

5.2 kb
7.9 kb
9.5 kb
2.5 kb

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Vectors Uses for Animal Cell Culture
Maximum insert size in case of adenovirus vectors is

20 kb
6-8 kb
10-12 kb
4-6 kb

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Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Galactokinase (GAL 1)
Alcohol dehydrogenase II (ADH 2)
All of these
Phosphoglycerate kinase (PGK)

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Vectors Uses for Animal Cell Culture
Which of the following promoters is repressed in presence of glucose?

Galactokinase (GAL 1)
Phosphoglycerate kinase (PGK)
Acid phosphatase (PHOS)
Both (a) and (b)

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Carries autonomously replicating sequence
Allows transformation by using crossing over
Has a origin of replication and rep genes
Carries centromere and telomere sequences

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

episomally only
either episomally or integrated at high copy number
either episomally or integrated at low copy number
integrated only

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