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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Bovine papilloma virus has a circular double stranded DNA genome of about

9.5 kb
5.2 kb
7.9 kb
2.5 kb

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3
Trp 1
His 3
Lys 2

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

SV 40 helper virus
pBR 322 vector
COS cell line
none of these

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

All of these
Ability to clone large piece of DNA
Over production of proteins of commercial value
Ability to glycosylate proteins during secretion

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Vectors Uses for Animal Cell Culture
Recombinant SV40 viruses introduce the foreign DNA into cells

with the DNA mediated transfection
without the RNA mediated transfection
with the RNA mediated transfection
without the DNA mediated transfection

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Vectors Uses for Animal Cell Culture
Which of the following are produced in high copy number?

YRp
YEp
Both (a) and (b)
YCp

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