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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following(s) is/are the most commonly used markers in yeast?

Trp 1
Ura 3
His 3
All of these

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Has a origin of replication and rep genes
Carries autonomously replicating sequence
Carries centromere and telomere sequences
Allows transformation by using crossing over

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Vectors Uses for Animal Cell Culture
YAC vectors can be used to obtain artificial chromosomes because it carries

all of these
telomere
autonomously replicating sequence
centromere

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Vectors Uses for Animal Cell Culture
Autonomously replicating sequence is not present in

YAC
YCp
YRp
YIp

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at high copy number
integrated only
episomally only
either episomally or integrated at low copy number

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Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

COS cell line
none of these
pBR 322 vector
SV 40 helper virus

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