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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Autonomously replicating sequence is not present in

YCp
YAC
YIp
YRp

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Vectors Uses for Animal Cell Culture
Cos cell line is

derivative of permissive CV-1 monkey cell line
cohesive initial site of phage λ
derivative of non-permissive CV-1 monkey cell line
cohesive end site of phage λ

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Vectors Uses for Animal Cell Culture
The size of the genome of SV 40 is

7.2 kb
110 kb
5.2 kb
69 kb

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Has a origin of replication and rep genes
Carries centromere and telomere sequences
Carries autonomously replicating sequence
Allows transformation by using crossing over

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Vectors Uses for Animal Cell Culture
Lytic infection of SV 40 does not occur in

none of these
non permissive cells
both (a) and (b)
permissive cells

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Ability to glycosylate proteins during secretion
Ability to clone large piece of DNA
All of these
Over production of proteins of commercial value

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