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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Autonomously replicating sequence is not present in

YIp
YCp
YAC
YRp

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Vectors Uses for Animal Cell Culture
Which of the following viruses are used as vectors for transferring genes to animal cells?

SV 40 virus
All of these
Bovine papilloma virus (BVP)
Retroviruses

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Vectors Uses for Animal Cell Culture
YAC vectors can be used to obtain artificial chromosomes because it carries

all of these
centromere
telomere
autonomously replicating sequence

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Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

centromere
telomere
both (a) and (c)
autonomously replicating sequence

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

integrated only
either episomally or integrated at low copy number
either episomally or integrated at high copy number
episomally only

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Vectors Uses for Animal Cell Culture
Recombinant SV40 viruses introduce the foreign DNA into cells

without the DNA mediated transfection
without the RNA mediated transfection
with the DNA mediated transfection
with the RNA mediated transfection

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