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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

10 kb
2.5 kb
5 kb
1 kb

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Integrative plasmids (YIp) vectors?

Carries autonomously replicating sequence
Has a origin of replication and rep genes
Carries centromere and telomere sequences
Allows transformation by using crossing over

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at high copy number
either episomally or integrated at low copy number
integrated only
episomally only

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Vectors Uses for Animal Cell Culture
Cos cell line is

derivative of permissive CV-1 monkey cell line
cohesive end site of phage λ
derivative of non-permissive CV-1 monkey cell line
cohesive initial site of phage λ

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Vectors Uses for Animal Cell Culture
Which of the following viruses are used as vectors for transferring genes to animal cells?

All of these
Retroviruses
SV 40 virus
Bovine papilloma virus (BVP)

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Vectors Uses for Animal Cell Culture
Which one of the following is the most stable vector?

YEp
YAC
YRp
YIp

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