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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following can be used for reducing the size of gene inserts containing introns?

Vaccinia virus
Baculo virus
none of these
Retro virus

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Vectors Uses for Animal Cell Culture
α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Lys 2
His 3
Trp 1
Ura 3

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Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

10 kb
5 kb
2.5 kb
1 kb

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Over production of proteins of commercial value
All of these
Ability to glycosylate proteins during secretion
Ability to clone large piece of DNA

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Vectors Uses for Animal Cell Culture
Yeast Replicating plasmids (YRp) carry

both (a) and (c)
autonomously replicating sequence
telomere
centromere

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Allows transformation by using crossing over
Carries centromere and telomere sequences
Has a origin of replication and rep genes
Carries autonomously replicating sequence

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