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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following can be used for reducing the size of gene inserts containing introns?

Vaccinia virus
Baculo virus
none of these
Retro virus

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Vectors Uses for Animal Cell Culture
Which of the following promoters is repressed in presence of glucose?

Phosphoglycerate kinase (PGK)
Galactokinase (GAL 1)
Acid phosphatase (PHOS)
Both (a) and (b)

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Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Alcohol dehydrogenase II (ADH 2)
Galactokinase (GAL 1)
Phosphoglycerate kinase (PGK)
All of these

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Vectors Uses for Animal Cell Culture
α-aminoadipate inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3
Trp 1
His 3
Lys 2

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Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

5 kb
2.5 kb
10 kb
1 kb

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Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

integrated only
either episomally or integrated at high copy number
either episomally or integrated at low copy number
episomally only

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