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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
Which of the following can be used for reducing the size of gene inserts containing introns?

Vaccinia virus
Retro virus
none of these
Baculo virus

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Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Over production of proteins of commercial value
All of these
Ability to glycosylate proteins during secretion
Ability to clone large piece of DNA

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Lys 2
His 3
Ura 3
Trp 1

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Vectors Uses for Animal Cell Culture
Which of the following viruses are used as vectors for transferring genes to animal cells?

All of these
SV 40 virus
Bovine papilloma virus (BVP)
Retroviruses

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Vectors Uses for Animal Cell Culture
Retroviruses consist of

Ds DNA genome
cDNA genome
RNA genome
Ss DNA genome

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Vectors Uses for Animal Cell Culture
Which one of the following is true about Yeast Episomal plasmids (YEp) vectors?

Carries centromere and telomere sequences
Allows transformation by using crossing over
Has a origin of replication and rep genes
Carries autonomously replicating sequence

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