Vectors Uses for Animal Cell Culture
Cloning genes in the yeast offer which of following advantage(s)?

Ability to glycosylate proteins during secretion
Over production of proteins of commercial value
Ability to clone large piece of DNA
All of these

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

not necessary for viral replication
coding for virus coat protein
transcriptional regulators and are essential for viral replication
important for packaging of viral DNA into viral head

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Vectors Uses for Animal Cell Culture
Yeast episomal plasmids (YEp) were constructed using

2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322
12μm yeast plasmid and E coli vector pMB 9
12μm yeast plasmid and E coli vector pBR 322

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