Vectors Uses for Animal Cell Culture
Using bovine papilloma virus (BPV) derived vectors, permanent cell lines can be obtained carrying a recombinant DNA

either episomally or integrated at high copy number
episomally only
integrated only
either episomally or integrated at low copy number

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Vectors Uses for Animal Cell Culture
In case of Baculoviruses, the foreign gene

is inserted randomly in viral genome
is inserted downstream of viral origin of replication
is inserted downstream of polyhedrin promoter
is inserted between the polyhedrin promoter and downstream sequences

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Vectors Uses for Animal Cell Culture
Yeast episomal plasmids (YEp) were constructed using

12μm yeast plasmid and E coli vector pBR 322
12μm yeast plasmid and E coli vector pMB 9
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pMB 9
2μm yeast plasmid, fragments of yeast nuclear DNA and E coli vector pBR 322

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