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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
YAC vectors can be used to obtain artificial chromosomes because it carries

all of these
autonomously replicating sequence
centromere
telomere

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Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Phosphoglycerate kinase (PGK)
Alcohol dehydrogenase II (ADH 2)
All of these
Galactokinase (GAL 1)

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Vectors Uses for Animal Cell Culture
Autographa California nuclear polyhedrosis virus that is used as a vector for animal cells is an example of

Retrovirus
Baculo virus
Papilloma virus
Vaccinia virus

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

both (a) and (b)
non essential for baculo virus replication
essential for baculo virus replication
major component of protein matrix in which viruses are embedded

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Vectors Uses for Animal Cell Culture
5-fluoro orotic acid inhibits the growth if one of the following marker is present in the yeast. That is

Ura 3
Lys 2
His 3
Trp 1

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Vectors Uses for Animal Cell Culture
Maximum amount of foreign DNA introduced into SV 40 viruses is

1 kb
5 kb
10 kb
2.5 kb

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