Vectors Uses for Animal Cell Culture
Recombinant SV40 viruses introduce the foreign DNA into cells

without the RNA mediated transfection
with the RNA mediated transfection
without the DNA mediated transfection
with the DNA mediated transfection

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Vectors Uses for Animal Cell Culture
Recombinant adeno virus can be created by deleting E1A/E 1B genes that are

transcriptional regulators and are essential for viral replication
coding for virus coat protein
important for packaging of viral DNA into viral head
not necessary for viral replication

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Vectors Uses for Animal Cell Culture
Retroviruses have advantage for being used as vector for animal cells because

viral gene expression is driven by strong promoters
they cover a wide host range
All of these
infection does not lead to cell death, infected cells produce virus over an indefinite period

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