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Vectors Uses for Animal Cell Culture

Vectors Uses for Animal Cell Culture
SV 40 vectors, in which late region replacements are done, require

none of these
pBR 322 vector
SV 40 helper virus
COS cell line

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Vectors Uses for Animal Cell Culture
Which of the following histone is not present in SV40 virus?

H3
H4
H2A
H1

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Vectors Uses for Animal Cell Culture
Polyhedrin protein is

major component of protein matrix in which viruses are embedded
essential for baculo virus replication
both (a) and (b)
non essential for baculo virus replication

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Vectors Uses for Animal Cell Culture
Which of the following is/are commonly used promoter of S. cerevisiae?

Phosphoglycerate kinase (PGK)
Galactokinase (GAL 1)
Alcohol dehydrogenase II (ADH 2)
All of these

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Vectors Uses for Animal Cell Culture
Maximum insert size in case of adenovirus vectors is

4-6 kb
20 kb
10-12 kb
6-8 kb

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Vectors Uses for Animal Cell Culture
Cos cell line is

cohesive initial site of phage λ
derivative of permissive CV-1 monkey cell line
derivative of non-permissive CV-1 monkey cell line
cohesive end site of phage λ

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